The CUET PG Plant Biotechnology exam for 2025 will be conducted between 13th May and 3rd June, as part of the CUET PG exam window. Candidates can download the official question paper, answer key, and complete solution PDF after the exam concludes. The exam assesses topics such as plant tissue culture, genetic engineering in plants, molecular markers, transgenic crops, genomics, and bioinformatics applications in plant research.

The format includes 75 objective questions to be attempted in 60 minutes, with +4 for correct answers and -1 for incorrect answers.

CUET PG Plant Biotechnology 2025 Question Paper with Solutions PDF

CUET PG Plant Biotechnology Question Paper with Solutions PDF Download PDF Check Solutions


Question 1:

Plants having half the somatic chromosome number than found in normal individual are called -

  • (A) Monoploid
  • (B) Haploid
  • (C) Aneuploids
  • (D) Monosomics

Question 2:

The production of haploids by anther and pollen culture was first demonstrated by -

  • (A) Maheswari and Guha
  • (B) Ravi and Chan
  • (C) Kasha and Kao
  • (D) Clausen and Cameron

Question 3:

Plants cannot absorb molecular nitrogen from the atmosphere because

  • (A) It has double bonds making it highly stable.
  • (B) It has triple bonds making it highly stable.
  • (C) Its abundance in atmosphere inhibits absorption.
  • (D) It has double bonds making it highly unstable.

Question 4:

Inducing the formation of various vegetative organs from cells or tissues in plant tissue culture is called -

  • (A) Somatic embryogenesis
  • (B) Dedifferentiation
  • (C) Organogenesis
  • (D) Somatic hybridization

Question 5:

Production of secondary metabolites require the use of -

  • (A) Cell suspension
  • (B) Solid agar medium
  • (C) Meristem
  • (D) Axillary bud

Question 6:

Media that contain some chemical with unknown chemical composition is called ____________ media.

  • (A) Synthetic
  • (B) Enrichment
  • (C) Complex
  • (D) Selective

Question 7:

For obtaining pure cultures of bacteria ____________ plate method is not used.

  • (A) Streak
  • (B) Spread
  • (C) Pour
  • (D) Dip

Question 8:

Select the correct statements regarding somaclonal variations

A. These variations can also result in unwanted traits.
B. Somaclones have been developed and proved advantageous in several crops.
C. These variations can be used to engineer novel traits.
D. Short term invitro callus and cell suspension, cultures results in somaclonal variation.

Choose the correct answer from the options given below :

  • (A) A, C and D Only
  • (B) A, B and C Only
  • (C) A and B Only
  • (D) A and D Only

Question 9:

In plant tissue culture formation of organ primordia-like shoot or root in callus cells is called -

  • (A) Dedifferentiation
  • (B) Redifferentiation
  • (C) Somatic embryogenesis
  • (D) Regeneration

Question 10:

Who is regarded as father of 'Plant Tissue Culture' (P. T. C.)?

  • (A) Gottlieb Haberlandt
  • (B) Theodor Schwann
  • (C) Friedrich J. Haberlandt
  • (D) Robert Koch

Question 11:

The first androgenic haploid plant product by anther culture was from -

  • (A) Soybean
  • (B) Datura
  • (C) Potato
  • (D) Barley

Question 12:

The process of combining cytoplasmic genomes of one parent with nuclear genome of other parent is called -

  • (A) Somatic hybridization
  • (B) Micropropagation
  • (C) Cybridization
  • (D) Regeneration

Question 13:

____________ Molecules of ATP are required to fix one molecule of nitrogen (N2) to 2NH3.

  • (A) 4
  • (B) 8
  • (C) 16
  • (D) 20

Question 14:

Symbiotic nitrogen fixing bacteria are found in -

A. Azolla
B. Gnetum
C. Anthoceros
D. Cycas
E. Riccia

Choose the correct answer from the options given below :

  • (A) A, C and D Only
  • (B) B, C and E Only
  • (C) C, D and E Only
  • (D) B, C and D Only

Question 15:

Important enzymes involved in nitrogen fixation are -

  • (A) Nitrogenase and peptidase
  • (B) Nitrogenase and hexokinase
  • (C) Hexokinase and dehydrogenase
  • (D) Nitrogenase and hydrogenase

Question 16:

'Pomato' a hybrid of potato and tomato was produced through-

  • (A) Shoot tip culture
  • (B) Anther culture
  • (C) Somatic hybridization
  • (D) Seed culture

Question 17:

Arrange the following steps in the process of nitrogen cycle in correct sequence:

A. Nitrogen fixation
B. Nitrification
C. Denitrification
D. Assimilation
E. Ammonification

Choose the correct answer from the options given below :

  • (A) A, B, C, D, E
  • (B) C, D, E, A, B
  • (C) A, B, D, E, C
  • (D) C, A, B, D, E

Question 18:

Virus free plants can be grown by : -

  • (A) Embryo culture
  • (B) Apical meristem culture
  • (C) Callus culture
  • (D) Organ culture

Question 19:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-II, B-IV, C-I, D-III
  • (B) A-II, B-III, C-IV, D-I
  • (C) A-III, B-II, C-I, D-IV
  • (D) A-IV, B-III, C-II, D-I

Question 20:

Arrange the following steps for plant tissue culture (P.T.C.) in correct sequence:

A. Selection of desired material and suitable nutrient media for P. T. C.
B. Inoculation of explants
C. Surface sterilization of explant
D. Transfer of growing cultures
E. Transfer of plantlets to soil in pots

Choose the correct answer from the options given below :

  • (A) A, B, C, D, E
  • (B) A, C, B, D, E
  • (C) A, C, E, B, D
  • (D) B, C, A, E, D

Question 21:

In nitrogen cycle, Ammonification is the process of generating ammonia from-

  • (A) Amino acids
  • (B) Nitrates
  • (C) Nitrites
  • (D) Nitrogen

Question 22:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-III, B-IV, C-II, D-I
  • (B) A-II, B-III, C-IV, D-I
  • (C) A-I, B-II, C-III, D-IV
  • (D) A-IV, B-III, C-I, D-II

Question 23:

Conversion of ammonia to nitrates is called -

  • (A) Ammonification
  • (B) Assimilation
  • (C) Nitrification
  • (D) Denitrification

Question 24:

Choose the correct statements regarding plant tissue culture -

A. Organogenesis is inducing the formation of various vegetative organs from cells or tissues.
B. Formation of mass of undifferentiation cells from callus redifferentiation.
C. Cytoplasmic hybrids are prepared by taking the nucleus from one parent and cytoplasm from both the parents.
D. Relative concentration of growth hormones play important role in organogenesis.

Choose the correct answer from the options given below :

  • (A) A, B, and C Only
  • (B) B, C and D Only
  • (C) A and B Only
  • (D) A, C and D Only

Question 25:

Full form of CRISPR, a term used in genome editing is -

  • (A) Clustered regularly inter spaced short palindromic repeats
  • (B) Cumulative routinely inter spaced short palindromic repeats
  • (C) Cumulative regularly inter spaced short palindromic repeats
  • (D) Clustered routinely inter spaced slight palindromic repeats

Question 26:

Choose the correct statements regarding homozygous diploid plants -

A. They are produced by doubling the chromosome number of haploids.
B. Doubling of chromosome enables the recessive traits to express too.
C. Chromosome doubling is done by Ozone treatment.
D. Chromosome doubling is done by colchicine treatment.

Choose the correct answer from the options given below:

  • (A) A, B and D Only
  • (B) A, C and D Only
  • (C) A, B and C Only
  • (D) A, and D Only

Question 27:

Transgenic plants developed by introducing Bt gene in crops like brinjal, maize, cotton etc. provide resistance to:

  • (A) insect pests
  • (B) viral infection
  • (C) heat
  • (D) fungal diseases

Question 28:

Which of the following converts nitrites to nitrates?

  • (A) Clostridium
  • (B) Nitrobacter
  • (C) Nitrosomonas
  • (D) Nitrococcus

Question 29:

San Noeum first successfully cultured gynogenic haploid plants from unfertilized ovaries of -

  • (A) Maize
  • (B) Barley
  • (C) Wheat
  • (D) Rice

Question 30:

Nitrogen fixation occurs with the help of symbiotic bacteria in -

A. Pea
B. Lettuce
C. Beans
D. Tomato
E. Black gram

Choose the correct answer from the options given below :

  • (A) A, B and C Only
  • (B) B, C and D Only
  • (C) B, C and E Only
  • (D) A, C and E Only

Question 31:

In biological nitrogen fixation conversion of dinitrogen molecule into ammonia is carried out by enzyme.

  • (A) Hydrogenase
  • (B) Dehydrogenase
  • (C) Nitrogenase
  • (D) Nitrate reductase

Question 32:

Choose the correct statements regarding cytology of haploids -

A. A haploid in Arabidopsis will have 5 chromosomes.
B. Haploids are found as bivalents at metaphase-I of meiosis.
C. Haploids are found as univalent at metaphase-I of meiosis.
D. The haploids in maize will have 10 chromosomes.
E. The haploids in maize will have 20 chromosomes.

Choose the correct answer from the options given below :

  • (A) A, B and C Only
  • (B) A, C and E Only
  • (C) A, C and D Only
  • (D) A, B and E Only

Question 33:

Haploids can be artificially produced by -

A. Colchicine doubling
B. X-ray treatment
C. Pollen culture
D. Distant hybridization
E. Infrared radiation

Choose the correct answer from the options given below :

  • (A) A, B, and C Only
  • (B) B, C, and E Only
  • (C) A, D, and E Only
  • (D) B, C, and D Only

Question 34:

Autonomously replicating circular extrachromosomal DNA is called -

  • (A) Recombinant DNA
  • (B) Cybrid
  • (C) Plasmid
  • (D) Yeast artificial chromosome

Question 35:

Nitrogen fixing cyanobacteria Anabaena is found in the root pockets of -

  • (A) Azolla
  • (B) Pistia
  • (C) Marsilea
  • (D) Salvinia

Question 36:

The cutting of DNA at specific locations became possible with the discovery of -

  • (A) Reverse transcriptase
  • (B) Restriction endonuclease
  • (C) Bacteriophage
  • (D) P. C. R.

Question 37:

Arrange the basic steps to develop G. M. O. (Genetically modified organisms) in sequence:

A. Transfer of DNA with desired genes to its progeny
B. Identification of DNA with desired genes
C. Introduction of the DNA into the host.
D. Maintenance of introduced DNA in the host.

Choose the correct answer from the options given below :

  • (A) A, B, C, D
  • (B) B, C, A, D
  • (C) B, A, D, C
  • (D) B, C, D, A

Question 38:

A microbial biocontrol agent which control butterfly caterpillars in plants is -

  • (A) Bacillus thuringiensis
  • (B) Streptococcus sps.
  • (C) Saccharomyces cerevisiae
  • (D) Trichoderma polysperum

Question 39:

What is the function of leghemoglobin present in root nodulus of leguminous plants?

  • (A) Inhibition of nitrogenase activity
  • (B) Removal of oxygen
  • (C) Nodule differentiation.
  • (D) Expression of nif gene

Question 40:

CRISPR-Cas 9 is a gene ____________ technique.

  • (A) sequencing
  • (B) labelling
  • (C) editing
  • (D) locating

Question 41:

Choose the correct statements regarding Agrobacterium tumefaciens -

A. It is a gram positive round shaped bacterium
B. It is a gram negative rod shaped bacterium
C. It is a photosynthetic spiral bacterium
D. It is also known as 'Natural genetic Engineer'
E. It is capable of naturally transferring DNA into plant genome

Choose the correct answer from the options given below :

  • (A) A, B and C Only
  • (B) B, D and E Only
  • (C) B, C and D Only
  • (D) A, D and E Only

Question 42:

Source organism of cry genes is -

  • (A) Bacillus thuringiensis
  • (B) Agrobacterium tumefacien
  • (C) Rhizobium
  • (D) Staphylococcus

Question 43:

Which of the following elements play key role in nitrogen fixation?

  • (A) Zinc
  • (B) Copper
  • (C) Molybdenum
  • (D) Manganese

Question 44:

Which of the following methods/tools is not used for introduction of recombinant DNA into host cell?

  • (A) Microinjection
  • (B) Denaturation
  • (C) Gene gun
  • (D) Heat shock method

Question 45:

Arrange the following steps in the process of somatic hybridization in correct sequence:

A. Plating of fused protoplasts
B. Selection of hybrid cells
C. Protoplast isolation and its treatment with fusion chemical.
D. Transfer of callus to differentiation medium
E. Selection of somatic hybrid plants

Choose the correct answer from the options given below :

  • (A) A, B, C, D, E
  • (B) C, A, B, D, E
  • (C) B, C, D, A, E
  • (D) A, C, B, D, E

Question 46:

The correct combination of somaclonal variant released as a new cultivar is -

  • (A) Barley - Andro
  • (B) Geranium - Velvet Rose
  • (C) Tomato - DAMA
  • (D) Sugarcane - Scarlet

Question 47:

K. J. Kasha and coworkers found that following the cross between Hordeum vulgare \(\times\) Hordeum bulbosum, chromosomes of H. bulbosum were eliminated in early zygotic division, so few days after pollination, embryos can be cultured to get haploids. This method is called as -

  • (A) Delayed pollination
  • (B) Distant hybridization
  • (C) Nucellus culture
  • (D) Androgenesis

Question 48:

Antisense RNA technique is used -

  • (A) To silence the gene expression
  • (B) To enhance the gene expression
  • (C) For cell mediated gene transfer
  • (D) For DNA fingerprinting

Question 49:

Using natural predators for the control of pathogens is known as ____________ control.

  • (A) Physical
  • (B) Biological
  • (C) Chemical
  • (D) Enzymatic

Question 50:

Optimum pH for protoplast culture is -

  • (A) 6.5 to 7.0
  • (B) 5.5 to 5.9
  • (C) 4.5 to 4.9
  • (D) 7.5 to 7.9

Question 51:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-II, B-III, C-IV, D-I
  • (B) A-III, B-IV, C-II, D-I
  • (C) A-II, B-I, C-IV, D-III
  • (D) A-III, B-IV, C-I, D-II

Question 52:

Prions are the -

  • (A) infections proteinaceous agents
  • (B) DNA without protein coat
  • (C) RNA without protein coat
  • (D) Protozoans

Question 53:

What is CPW in protoplast culture method?

  • (A) Cell and protoplast washing
  • (B) Cytosol and protoplasm washing
  • (C) Cell and protoplast waste
  • (D) Cell and proteolytic waste

Question 54:

Arrange the following events in Western Blotting experiment in correct order:

A. Protein resolution by PAGE
B. Primary antibody binding
C. Transfer onto nitrocellulose membrane
D. Protein denaturation in loading dye.

Choose the correct answer from the options given below :

  • (A) A, C, B, D
  • (B) D, A, C, B
  • (C) B, A, D, C
  • (D) C, B, D, A

Question 55:

In cyanobacteria nitrogen fixation takes place in -

  • (A) Heterocyst
  • (B) Akinetes
  • (C) Nodules
  • (D) Hormogonia

Question 56:

Synthetic seeds are produced by the encapsulation of somatic embryos with-

  • (A) Sodium acetate
  • (B) Sodium chloride
  • (C) Sodium nitrate
  • (D) Sodium alginate

Question 57:

In plant tissue culture higher concentration of cytokinin generally promotes-

  • (A) Root regeneration
  • (B) Shoot regeneration
  • (C) Leaf primordia
  • (D) Flower initiation

Question 58:

Chemical most widely used for chromosome doubling in haploid culture is-

  • (A) Sorbitol
  • (B) Mannose
  • (C) Colchicine
  • (D) Mannitol

Question 59:

Which combination of strategies forms the basis of in vivo haploid induction technologies in plants?

A. induction of parthenogenesis
B. culture of anthers or ovules
C. use of paternal inducer lines
D. uniparental genome elimination

Choose the correct answer from the options given below:

  • (A) A, B and C only
  • (B) B, C and D only
  • (C) A, C and D only
  • (D) B, and D only

Question 60:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-II, B-III, C-IV, D-I
  • (B) A-III, B-IV, C-I, D-II
  • (C) A-I, B-III, C-II, D-IV
  • (D) A-I, B-II, C-III, D-IV

Question 61:

Select the correct combination of protoplast isolation enzyme and its most popular source -

  • (A) Cellulase - Helix pomatia
  • (B) Hemicellulase - Tricoderma viride
  • (C) Macerozyme R - 10 - Rhizopus arrhizus
  • (D) Zymolyase - Aspergillus niger

Question 62:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-IV, B-II, C-III, D-I
  • (B) A-II, B-IV, C-III, D-I
  • (C) A-II, B-III, C-IV, D-I
  • (D) A-III, B-II, C-I, D-IV

Question 63:

Copies of DNA strands generated during a polymerase chain reaction are known as -

  • (A) Multicons
  • (B) Polycons
  • (C) Amplicons
  • (D) Monocons

Question 64:

Which gene in shoot apical meristem (SAM) negatively regulate WUS expression?

  • (A) CLV
  • (B) STM
  • (C) AP1
  • (D) TAB1

Question 65:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-I, B-II, C-IV, D-III
  • (B) A-II, B-III, C-IV, D-I
  • (C) A-II, B-I, C-IV, D-III
  • (D) A-IV, B-III, C-II, D-I

Question 66:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-II, B-III, C-IV, D-I
  • (B) A-III, B-IV, C-I, D-II
  • (C) A-III, B-IV, C-II, D-I
  • (D) A-I, B-II, C-III, D-IV

Question 67:

In general, the cytoplasmic male sterility (CMS) causing genes are transcribed in which plant cell organelle?

  • (A) Nucleus
  • (B) Peroxisome
  • (C) Mitochondria
  • (D) Chloroplast

Question 68:

Match the LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-II, B-III, C-IV, D-I
  • (B) A-III, B-IV, C-I, D-II
  • (C) A-IV, B-III, C-II, D-I
  • (D) A-I, B-II, C-III, D-IV

Question 69:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-II, B-III, C-IV, D-I
  • (B) A-III, B-II, C-I, D-IV
  • (C) A-III, B-IV, C-I, D-II
  • (D) A-I, B-II, C-III, D-IV

Question 70:

Match the LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-I, B-II, C-III, D-IV
  • (B) A-I, B-III, C-II, D-IV
  • (C) A-III, B-I, C-IV, D-II
  • (D) A-III, B-IV, C-I, D-II

Question 71:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-II, B-III, C-IV, D-I
  • (B) A-III, B-IV, C-II, D-I
  • (C) A-II, B-IV, C-I, D-III
  • (D) A-I, B-II, C-III, D-IV

Question 72:

Which of the following will be present in the F1 multicellular embryo, derived from a cross of female plant (A) with male plant B, through "bulbosum method"?

  • (A) Chromosomes of A
  • (B) Homologous chromosomes of A and B
  • (C) Chromosomes of B
  • (D) recombinant chromosomes of A and B

Question 73:

Arrange the steps in PEG induced protoplast fusion in correct sequence -

A. Treatment of protoplast mixture with 28-50% PEG for 15-30 minutes.
B. Protoplast aggregation.
C. Washing of protoplast (alkaline medium pH 9-10), and high Ca2+ concentration
D. Selection of protoplasts of different strains / species.

Choose the correct answer from the options given below :

  • (A) A, B, C, D
  • (B) D, A, B, C
  • (C) C, D, B, A
  • (D) D, B, C, A

Question 74:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-III, B-II, C-IV, D-I
  • (B) A-I, B-II, C-IV, D-III
  • (C) A-III, B-IV, C-II, D-I
  • (D) A-II, B-III, C-IV, D-I

Question 75:

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

  • (A) A-III, B-IV, C-II, D-I
  • (B) A-III, B-IV, C-I, D-II
  • (C) A-II, B-IV, C-I, D-III
  • (D) A-III, B-II, C-IV, D-I