The question paper and solution PDF of the GATE BT (Biotechnology) paper is available here. The exam ‌for BT paper was scheduled for 7th February, session 1 (2:30 PM to 5:30 PM). 

The Gate 2026 BT exam happens for 100 marks, where 15 marks come from the general aptitude section, 13 marks from Engineering mathematics. The remaining 72 marks come from core subjects. 

Based on the intitial analysis, the GATE 2026 BT question paper was considered moderate to tough. To rank under 1000 in Instrumentation, you need to score 55-60+ marks out of 100. 

GATE 2026 BT Question Paper with Solution PDF (Memory- Based)

GATE 2026 Biotechnology (BT) Question Paper with Solution PDF Download Check Solutions

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Question 1:

Which of the following bonds is responsible for maintaining the secondary structure of proteins?

  • (A) Peptide bond
  • (B) Hydrogen bond
  • (C) Disulfide bond
  • (D) Ionic bond

Question 2:

Which microorganism is commonly used for industrial production of ethanol?

  • (A) Escherichia coli
  • (B) Bacillus subtilis
  • (C) Saccharomyces cerevisiae
  • (D) Lactobacillus

Question 3:

Which of the following is a recessive genetic disorder?

  • (A) Huntington's disease
  • (B) Sickle cell anemia
  • (C) Achondroplasia
  • (D) Marfan syndrome

Question 4:

Which parameter is most commonly controlled in a bioreactor?

  • (A) Light intensity
  • (B) pH
  • (C) Pressure
  • (D) Sound

Question 5:

Which enzyme is responsible for synthesis of RNA from a DNA template?

  • (A) DNA polymerase
  • (B) RNA polymerase
  • (C) Reverse transcriptase
  • (D) DNA ligase

Question 6:

Which antibody class is most abundant in human serum?

  • (A) IgA
  • (B) IgD
  • (C) IgE
  • (D) IgG

Question 7:

The most commonly used technique for separation of proteins is:

  • (A) Centrifugation
  • (B) Chromatography
  • (C) Filtration
  • (D) Precipitation

GATE 2026 Question Paper