CBSE Class 12 Biology Set 1 Question Paper PDF 57-2-1 is now available for download. CBSE conducted the Class 12 Biology examination on March 25, 2025. The question paper consists of 33 questions carrying a total of 70 marks. Section A includes 16 MCQs for 1 mark each, Section B contains 5 very short-answer questions for 2 marks each, Section C comprises 7 short-answer questions for 3 marks each, Section D comprises 2 Case-based questions carries 4 marks each and Section E comprises 3 long-answer questions carries 5 marks each.
CBSE Class 12 2025 Biology Question Paper 57-2-1 with Answer Key
| CBSE Class 12 2025 Biology Question Paper with Answer Key | Download PDF | Check Solution |

Given below is a diagram of T.S. of a monocot seed with parts I, II \& III labelled :
Choose the option where parts I, II and III are identified correctly.
The number of autosomes present in a human secondary spermatocyte
A child with blood group A has the father with blood group B and the mother with blood group AB. Choose the option that gives the correct genotypes of father, mother and the child :
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In a pedigree chart represents :
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Step 1: In a pedigree chart, a circle typically represents a female and a square represents a male.
Step 2: A horizontal line connecting them with a specific symbol, such as \(\bigcirc \! \! \! \! \! \! = \! \! \! \! \! \! \square\), indicates a consanguineous mating, meaning mating between relatives.
Step 3: This symbol does not represent unrelated mating, affected individuals, or twins.
Thus, the correct interpretation is mating between relatives (consanguineous mating).
Quick Tip: In pedigree charts, consanguineous mating is often indicated by a double line or specific notation between the symbols for male and female.
Which one of the following options shows the correct evolutionary order of the plants mentioned below?
(i) Ferns \quad (ii) Ginkgo \quad (iii) Zosterophyllum \quad (iv) Gnetales
Choose the correct option.
The phosphoester linkage in the nucleotides is between
Given below is a heterogeneous RNA formed during Eukaryotic transcription:
How many introns and exons respectively are present in the hnRNA?
Study the items of Column-I and those of Column-II:
Column-I \quad \quad \quad \quad \quad \quad \quad \quad Column-II
(a) RNA polymerase I \quad \quad \quad (i) 18s rRNA
(b) RNA polymerase II \quad \quad (ii) SnRNAs
(c) RNA polymerase III \quad \quad (iii) hnRNA
Choose the option that correctly matches the items of Column-I with those of Column-II:
For commercial and industrial production of citric acid, which one of the following microbes is used?
If Meselson and Stahl's experiment is continued for 80 minutes (till III generation), what would be the ratio of DNA containing N\(^{15}\)/N\(^{15}\) : N\(^{15}\)/N\(^{14}\) : N\(^{14}\)/N\(^{14}\) in the medium?
Select the correct statement from the following biotechnological procedures:
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The decrease in the T-Lymphocytes count in human blood will finally result in
Assertion (A): Corpus luteum secretes the hormone, progesterone.
Reason (R): Hormone Progesterone is essential for maintenance of the endometrium.
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Assertion (A): The number of white winged moths decreased after industrialisation in England.
Reason (R): Effects of industrialisation were more marked in rural areas of England.
Assertion (A): Streptococcus pneumoniae and Haemophilus influenzae are responsible for causing infectious disease in human beings.
Reason (R): A healthy person acquires the infection by inhaling the aerosols released by an infected person.
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Assertion (A): Restriction endonuclease recognises palindromic sequence in the DNA and cuts them.
Reason (R): Palindromic sequence has two unique recognition sites PstI and PvuI recognised by the restriction endonuclease.
Student to attempt either option (A) or (B):
(A)
(i) Write two crucial changes, the seed undergoes while reaching maturity that enable them to be in a viable state until the onset of favourable conditions.
(ii) Name the oldest viable seed excavated from Arctic Tundra as per the records.
\textbf{OR}
(B)
(i) Pea flower produced seed sets. Give reason.
(ii) In case of Polyembryony, an embryo ‘P’ develops from a synergid and the embryo ‘Q’ develops from the nucellus. State the ploidy of embryo ‘P’ and ‘Q’.
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Study the given pedigree chart in which neither of the parents shows the trait but the trait is present in both male and female children.
Answer the following questions:
(a) Write the trait, also explain the inheritance of such trait in the progeny on the basis of given pedigree chart.
(b) Give one example of such trait in human beings.
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Student to attempt either option (A) or (B).
(A) Describe any two situations where a medical doctor would recommend injection of pre-formed antibodies (antitoxins) into the body of a patient.
\textbf{OR}
(B) The symptoms of malaria do not appear immediately after the entry of sporozoites into the human body when bitten by female Anopheles mosquito. Explain why it happens.
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Observe the given sequence of nitrogenous bases on a DNA fragment and answer the following questions:
(a) Name the restriction enzyme which can recognise the DNA sequence.
(b) Write the sequence after restriction enzyme cut the palindrome.
(c) Why are the ends generated after digestion called as ‘Sticky Ends’?
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Student to attempt either option (A) or (B).
(A) Identify the type of pyramid given below and write two identifying features of such a pyramid:
\textbf{OR}
(B)
(i) Construct an ideal pyramid of energy when 10,00,000 Joules of sunlight is available.
(ii) Mention the energy obtained by the fourth level of this pyramid.
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(a) A bilobed dithecous anther has 200 microspore mother cells per microsporangium. How many male gametophytes can be produced by this anther?
(b) Write the composition of intine and exine layers of a pollen grain.
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(a) List two reasons that make copper releasing IUDs as effective contraceptives.
(b) Explain how the intake of oral contraceptive pills prevent pregnancy in humans.
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Using a Punnett square workout the distribution of an autosomal phenotypic feature in the first filial generation after a cross between a homozygous female and a heterozygous male for a single locus.
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How does the process of Natural Selection affect Hardy-Weinberg equilibrium? Explain with the help of graphs.
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Samples of blood and urine of a sportsperson are collected before any sports event for drug tests.
(a) Why there is a need to conduct such tests?
(b) Name the drugs the authorities usually look for.
(c) Write the generic names of two plants from which these drugs are obtained.
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(a) The insulin synthesised in our body is different from that synthesised by Eli Lilly company using recombinant DNA technology. Differentiate between them.
(b) Why the insulin extracted from an animal source is not in use these days?
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(a) Draw a graph for a population whose population density has reached the carrying capacity.
(b) Out of the two population growth curves, which one is considered a more realistic for most populations? Why?
(c) Draw a growth curve where resources are not limiting for the growth of a population and give its equation.
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Immunity in our body is of two types: (i) Innate immunity and (ii) acquired immunity. Innate immunity is a non-specific defence mechanism, whereas acquired immunity is pathogen-specific; it is called specific immunity too. Acquired immunity is characterised by memory. Antibodies are specific to antigens and there are different types of antibodies produced in our body: they are IgA, IgE, IgG and IgM. It shows primary response when it encounters the pathogen for the first time and secondary response during the subsequent encounters with the same Antigen/Pathogen.
(a) Name the two types of specialised cells which carry out the primary and secondary immune response.
(b) Why is the antibody-mediated immunity also called as humoral immune response?
Attempt either sub-part (c) or (d):
(c) The organ transplants are often rejected if taken from suitable compatible persons.
(i) Mention the characteristic of our immune system that is responsible for the graft rejection.
(ii) Name the type of immune response and the cell involved in it.
\textbf{OR}
(d) How is active immunity different from passive immunity?
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The process of copying the genetic information from one strand of DNA into RNA is termed as transcription. The principle of complementarity of bases governs the process of transcription, also except that uracil comes in place of thymine.
Study the complete transcription unit given below and answer the following questions:
(a) Name the main enzyme involved in the process of transcription.
(b) Identify coding strand and template strand of DNA in the transcription unit.
Attempt either sub-part (c) or (d):
(c) Identify (C) and (D) in the diagram, mention their significance in the process of transcription.
OR
(d) Describe the location of (C) and (D) in the transcription unit.
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Student to attempt either option (A) or (B).
(A)
(i) Describe the process of megasporogenesis in an angiosperm.
(ii) Draw a diagram of a mature embryo sac of the angiosperm. Label its any four parts.
\textbf{OR}
(B) The reproductive cycle in the female primates is called menstrual cycle. The first menstruation begins at puberty.
Answer the following questions:
(i) Name the four phases of menstrual cycle in a proper sequence.
(ii) How long does the menstrual phase last in a menstrual cycle?
(iii) When and why hormones estrogen and progesterone reach their peak levels respectively, in the menstrual cycle?
(iv) Give the significance of LH surge.
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Student to attempt either option (A) or (B).
(A)
(i) Explain how is a bacterial cell made ‘competent’ to take up recombinant DNA from the medium.
(ii) Explain the steps of amplification of gene of interest using PCR technique.
\textbf{OR}
(B)
(i) What are transgenic animals?
(ii) Why are these animals being produced? Explain any four reasons.
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Student to attempt either option (A) or (B).
(A)
(i) Explain giving three reasons why tropics show greatest levels of species diversity.
(ii) Draw a graph showing species-area relationship. Name the naturalist who studied such relationship. Write the observation made by him.
\textbf{OR}
(B)
(i) The world is facing the accelerated rate of species extinctions due to human activities. Explain any three major causes of biodiversity losses.
(ii) Describe ‘Ex situ’ approach for conserving biodiversity. Give any two examples.
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