Biotechnology - NEET Previous Year Questions with Solutions and Weightage

Biotechnology is an important and high-weightage topic that is asked in the NEET UG 2025 exam. The topic carries around 8% weightage and candidates can expect 3 to 4 questions from this section in the NEET 2025 exam.

Biotechnology is an essential part of NEET 2025 Biology that will help you to understand the Principles and process of Biotechnology: Genetic engineering, Application of Biotechnology in health and agriculture: Human insulin and vaccine production, gene therapy; Genetically modified organisms-Bt crops; Transgenic Animals; Biosafety issues-Biopiracy and patents. With a weightage of 8-9%, Diversity in the living world is one of the most essential topics in NEET Biology Syllabus and understanding it will help you fetch around 14-15 marks.

Must Read Articles:

• Download NEET Last 10 year PYQ for Free
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Listed below is the list of Biotechnology questions with detailed solutions asked in NEET UG 2025 exam.

Question 1:

The following diagram shown restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’gens :

• The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.
• The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.
• The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics
• Gene ’X’ is responsible for recognitions sites and ‘Y’ is responsible for antibiotic resistance

View Solution

Question 2:

Which of the following statements is incorrect?

• A bio-reactor provides optimal growth conditions for achieving the desired product
• Most commonly used bio-reactors are of stirring type
• Bio-reactors are used to produce small scale bacterial cultures
• Bio-reactors have an agitator system, an oxygen delivery system foam control system

View Solution

Question 3:

The “Ti plasmid” of Agrobacterium tumefaciens stands for

• Tumour inhibiting plasm
• Tumor independent plasmid
• Tumor inducing plasmid
• Temperature independent plasmid

View Solution

Question 4:

The capacity to generate a whole plant from any cell of the plant is called:

• Totipotency
• Micropropagation
• Differentiation
• Somatic hybridization

View Solution

Question 5:

Match List-I with List-II:

Choose the correct answer from the options given below:

• A-IV, B-I, C-III, D-II
• A-I, B-IV, C-II, D-III
• A-III, B-I, C-IV, D-II
• A-I, B-II, C-IV, D-III

View Solution

Question 6:

Match List-I with List-II:

Choose the correct answer from the options given below:

• A-II, B-III, C-IV, D-I
• A-III, B-II, C-I, D-IV
• A-II, B-I, C-IV, D-III
• A-II, B-I, C-III, D-IV

View Solution

Question 7:

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector

• Bam HI and Sal I
• Sal I and Pst I
• Pst I and Pvu I
• Pvu I and Bam HI

View Solution

Question 8:

Following are the steps involved in action of toxin in Bt. Cotton

A. The inactive toxin converted into active form due to alkaline pH of gut of insect.

B. Bacillus thuringiensis produce crystals with toxic insecticidal proteins.

C. The alkaline pH solubilises the crystals.

D. The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect.

E. The toxin proteins exist as inactive protoxins in bacteria.

Choose the correct sequence of steps from the options given below:

• E → C → B → A → D
• B → C → A → E → D
• A → E → B → D → C
• B → E → C → A → D

View Solution

Question 9:

The Bt toxin in genetically engineered Bt cotton kills the pest by:

• Creating pores in the midgut
• Damaging the respiratory system
• Degenerating the nervous system
• Altering the pH of body fluids

View Solution

Question 10:

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

• Hind II, Hind II
• Hind II, Alu I
• Hind II, EcoR I
• Hind II, BamHI

View Solution

Question 11:

Given below are two statements:

Statement I : The Indian Government has set up GEAC, which will make decisions regarding the validity of GM research.

Statement II : Biopiracy is the term used to refer to the use of bio-resources by native people.

In the light of the above statements, choose the correct answer from the options given below :

• Statement I is true but Statement II is false
• Statement I is false but Statement II is true
• Both Statement I and Statement II are true
• Both Statement I and Statement II are false

View Solution

Question 12:

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

• Insect pests
• Fungal diseases
• Plant nematodes
• Insect predators

View Solution

NEET 2025 Biology Exam Pattern

The Biology section in NEET 2025 plays a pivotal role in determining the overall score, given its significant weightage in the exam. Comprising 90 questions out of the total 180, Biology is divided into two subsections: Botany and Zoology. There will be a total of 45 questions in each.

As per the marking scheme, +4 marks will be awarded for correct answers, -1 for incorrect ones, and unattempted questions will not be marked. Time duration for the exam will be 180 minutes.

Each subsection evaluates the aspirant’s knowledge across a wide spectrum of topics from Classes 11 and 12 NCERT syllabi.

NEET Biology Exam Pattern: Section-Wise Marking Scheme

The NEET 2025 Biology section comprises 90 questions, equally divided between Zoology (45 questions) and Botany (45 questions).

The marking scheme awards +4 marks for each correct answer, -1 mark for incorrect answers, and 0 marks for unattempted questions. The Biology section carries a total of 360 marks, split equally between Zoology and Botany. The exam is conducted offline (pen-paper mode).

Class-Wise Biology NEET Syllabus Weightage

Biology NEET Syllabus Weightage

The topic-wise weightage for the syllabus of Biology is provided here based on previous year exam:

Biology NEET 2025 Syllabus PDF- Chapter-Wise Weightage

NEET 2025 Chapter wise Weightage Biology Class 11

NEET 2025 Chapter wise Weightage Biology Class 12

Biology NEET Questions PDF

To practice subject-specific questions for NEET exam preparation, check NEET Biology questions from the link given below.

Biology NEET Exam Pattern 2024

Biology NEET exam pattern 2024 is provided below with the number of questions, type of questions, total marks, and negative marking.

NEET Question Paper 2024 PDF with Answer Key and Solution

NEET 2024 Question Paper PDF Code R

NEET 2024 Question Paper PDF Code Q

NEET 2024 Question Paper PDF Code T

NEET 2024 Question Paper PDF Code S

NEET Re-Exam June 23 Question Paper

NEET Question Paper 2024

NEET Subject Wise Question Paper 2024 PDF with Answer Key and Solution

NEET Sample Paper 2024 PDF Download with Answer

Students must not confuse this sample paper with the actual question paper. This is just a mock paper with answers that will help you with your exam preparation.

Frequently Asked Questions:

Q1. Is biotechnology important for the NEET 2025 exam?

Yes, biotechnology is important for the NEET exam, as it contributes about 8-9% of the overall Biology weightage in NEET, which is about 4-5 questions in the exam.

Topics like Application of Biotechnology in health and agriculture, gene therapy; Genetically modified organisms-Bt crops; Transgenic Animals; Biosafety issues-Biopiracy and patents.

Q2. What is the toughest chapter in biology for NEET 2025?

The most challenging chapters in Biology for NEET have the following topics like Genetics and Evolution, Human Physiology, and Plant Physiology particularly challenging, requiring focused study and practice.

NEET 2025 Application Process Latest News

• NEET Exam Dates 2025(Soon)
• NEET UG 2025: NTA Announces Exam Details and Guidelines, Download PDF Now

Q3. Are PYQs repeated in NEET exam?

No, PYQs are not repeated as such in NEET. Whereas, the concept and nature of questions from previous years tend to return in some other form.

• NEET generally attempts to test the same fundamental subjects every year, so PYQs make you familiar with the pattern of the exam and commonly tested concepts.
• While the precise questions are not recycled, the difficulty, question style, and conceptual emphasis are frequently the same.

Q4. Biotechnology: Genetic Engineering & Applications NEET 2025 weightage

The Biotechnology in the NEET holds around 8% weightage, which corresponds to about 3-4 questions in the exam.

Genetic Engineering and applications is the part of Biotechnology topic, the topics like Genetic engineering, Human insulin and vaccine production, gene therapy; Genetically modified organisms-Bt crops; Transgenic Animals ;are important to understand.

Q5. Biotechnology and Genetic Engineering Notes

Biotechnology and Genetic Engineering is an important concept in biotechnology for NEET 2025 exam. Biotechnology is a branch of biology dealing with techniques of using living organisms or parts of it to develop products helpful for human beings. The European Federation of Biotechnology (EFB) uniquely defines it as “the integration of natural science and organisms, cells, parts thereof, and molecular analogues for products and services”.

• This topic is of particular interest to all medical aspirants. At least 4-5 questions can be expected from this section in NEET 2025.
• Pretty easy questions are framed from this section and you can score well in this section.